Coding

Part:BBa_K5115000:Design

Designed by: Yi Shi   Group: iGEM24_Fudan   (2024-08-17)


RcnR_C35L


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have codon-optimized the original sequence based on E. coli K12 to enable its expression in Escherichia coli.


Source

synthetic


References